Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids
ثبت نشده
چکیده
For many years, analysis of short chain fatty acids (volatile fatty acids, VFAs) has been routinely used in identification of anaerobic bacteria. In numerous scientific papers, the fatty acids between 9 and 20 carbons in length have also been used to characterize genera and species of bacteria, especially nonfermentative Gram negative organisms. With the advent of fused silica capillary columns (which allows recovery of hydroxy acids and resolution of many isomers), it has become practical to use gas chromatography of whole cell fatty acid methyl esters to identify a wide range of organisms.
منابع مشابه
Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids
For many years, analysis of short chain fatty acids (volatile fatty acids, VFAs) has been routinely used in identification of anaerobic bacteria. In numerous scientific papers, the fatty acids between 9 and 20 carbons in length have also been used to characterize genera and species of bacteria, especially nonfermentative Gram negative organisms. With the advent of fused silica capillary columns...
متن کاملOptimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids.
Gas-liquid chromatography of cellular fatty acids was used in automatic identification of clinical bacterial isolates. The intraspecies variation in the occurrence of fatty acids and the variation in the relative gas-liquid chromatography peak areas of different fatty acids were evaluated and compared with the relative peak areas of these acids. A new chromatogram comparison method involving th...
متن کاملDetermination and study the fatty acid contents and their seasonal variations by temperature of a dominant bivalve(Callista umbonella) of Haleh Creek
In this study fatty acid contents, because of the importance in human’s life, and their seasonal variations of a dominant bivalve of Haleh Creek were determined for the first time. Fatty acid identification was done by GC-MS (Gas Chromatography–Mass Spectrometry) method. After collecting and dissecting the species from the shells, samples weighed and frozen for further experiments. All the samp...
متن کاملCellular fatty acids in Fusobacterium species as a tool for identification.
Identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests because of the relative biochemical inactivity of these bacteria. Even the finding of pointed, thin gram-negative cells on Gram-stained slides can no longer be relied on for identification of Fusobacteriu...
متن کاملBacterial Identification by Gas Chromatographic Analysis of Fatty Acid Methyl Esters (GC-FAME)
For many years, analysis of short chain fatty acids (volatile fatty acids, VFAs) has been routinely used in identification of anaerobic bacteria. In numerous scientific papers, the fatty acids between 9 and 20 carbons in length have also been used to characterize genera and species of bacteria, especially nonfermentative Gram negative organisms. With the advent of fused silica capillary columns...
متن کامل